Interdiscip Sci Comput Life Sci (2009) 1: 81–90
DOI: 10.1007/s12539-009-0036-7
Electromagnetic Signals Are Produced by Aqueous Nanostructures
Derived from Bacterial DNA Sequences
Luc MONTAGNIER1,2∗,….
Recevied
3 January 2009 / Revised 5 January 2009 /
Accepted 6 January 2009
= !!!!!!
a microorganism of about 300 nM in size, through filters of 100 nM or 20 nM porosities,
=комментарии излишни – во всей статье размеры – в единицах молярной концентрации
In addition to M. pirum, a more classical bacterium, E. Coli,
=то же самое, кэплок не в тему
Each dilution is done in 1.5 mL Eppendorf plastic tubes, which are then tightly stoppered and strongly agitated on a Vortex apparatus for 15 seconds . This step has been found critical for the generation of signals.
= гомеопатия воды
The original unfiltered suspension was negative at all dilutions, a phenomenon observed for all the microorganisms studied.
= все дело в фильтрах ?
Size and density of the structures producing the signals in the aqueous dilutions:
An aliquot of the 20 nM filtrate was layered on the top of a 5-20% (w/v) sucrose gradient in water and centrifuged for 2 hours at 35,000 rpm in a swinging bucket rotor. These conditions had previously been used to obtain the density equilibrium of the intact mycoplasma cells wich formed a sharp bound at 1,21 density. Fractions were collected from the bottom of the tubes, pooled 2 by 2 and assayed for signal emission. Fig. 4 shows that the signal emitting structures were distributed in a large range of densities from 1.15 to 1.25 and also had a high sedimentation coefficient.
Fig. 4 Sucrose density centrifugation (35 000 rpm, 2 Hr) of a 0.02μ filtrate of Mycoplasma pirum suspension. The collected fractions were pooled 2 by 2 and diluted up to D-15 and tested for EMS. The bars indicate the fractions positive for EMS.
= опять небрежность в написании единиц никогда не видел чтоб «часы» обозначали как ( Hr )
10,000 rpm for15minutes, the supernatant was filtered on 450nM filter and the resulting filtrate was
filtered again on a 100nM filter Signal producing dilutions usually range from 10−8 to 10−12,
=исходно взято 109 клеток E. Coli (1 мл в 1.5 мл пробирке). Т.е., изначально фильтровали супернатант питательной среды, а потом просто воду..
The only difference with M. pirum was that no signal appeared after filtration on 20 nM filters, suggesting that the structures associated with the signals were retained by these filters and, therefore, had a size greater than 20 nM and lower than 100 nM.
When we added 0.1 mL of a negative low dilution (e.g. 10−3) to 0.4 mL or 0.9 mL of a positive dilution (10−8), the latter became negative. This indicate that the ”silent” low dilutions are self-inhibitory, probably by interference of the multiple sources emitting in the same wave length or slightly out of phase, like a radio jamming.
A donor tube of a low “silent” dilution of E. Coli (10−3) was placed side by side close to a receiver tube of the positive “loud” highest dilution of the same preparation (10−9). Both tubes were placed in a mumetal box for 24 hours at room temperature, so that the tubes were not exposed to external electromagnetic noise, and only exposed to the signals generated by the structures present in the tubes themselves.
The tubes were then read again by the signal detecting device: the donor tube was still silent, however the receiver tube became also silent. Moreover, when further dilutions were made from the receiver tube (10−10, 10−11, 10−12), these dilutions had became positive (Fig. 6). These results suggest that the receiver tube was made silent by formation of an excess of new nanostructures, which could emit signals upon further dilution.
We then wonder whether or not it was possible to generate new signal-emitting structures from tube to tube by using wave transfer. A donor tube of a low “silent” dilution of E. Coli (10−3) was placed side by side close to a receiver tube of the positive “loud” highest dilution of the same preparation (10−9). Both tubes were placed in a mumetal box for 24 hours at room temperature, so that the tubes were not exposed to external electromagnetic noise, and only exposed to the signals generated by the structures present in the tubes themselves.
= Т.е., дополнительно ничем не облучались, а просто между собой «общались» по телепатски
The tubes were then read again by the signal detecting device: the donor tube was still silent, however the receiver tube became also silent. Moreover, when further dilutions were made from the receiver tube (10−10, 10−11, 10−12), these dilutions had became positive (Fig. 6). These results suggest that the receiver tube was made silent by formation of an excess of new nanostructures, which could emit signals upon further dilution.
Fig. 6 Cross-talk between dilutions (from an E. Coli 0.1 μ filtrate), see explanation in the text.
Importantly, the transfer effect between two tubes, one silent, one loud, was only observed if both contained dilutions of the same bacterial species. In other words, a Staphylococcus donor tube could only “talk” with a receiver tube containing a Staphylococcus dilution, and not with a tube of Streptococcus or E. Coli, and reciprocally. These results indicate that the transfer effect is mediated by species-specific signals,
For this a stationary culture of E. Coli was counted and adjusted to 109 cells/mL and serial dilutions from 100 to 100 were done down to 1 cell/mL. Each dilution was filtered at 100 nM and then analyzed for signal emission. Surprisingly, the range of positive dilutions were not strictly dependent on the initial concentration of E. Coli cells, being roughly the same from 109 cells down to 10 cells, suggesting that the same final number of nanostructures was reached at all concentrations. Thus, paradoxically, 10 cells are giving the same signals than 109 cells.
= без комментариев. Точнее, источник ЕМС – в самих фильтрах…
We were also concerned by the possible personal influence of the operator in the reading. To address this point, two healthy operators were asked to measure independantly the same dilutions of E. Coli, each one unknowing the results of the other. The results of their readings were identical.
We also found that the results were also independant of the location of the reading site: starting from the same unfiltered preparation of E. Coli, positive dilutions of the filtrates were found to be the same in two different locations in France (Paris center and suburb), one in Canada (Montreal), and one in Cameroun (Yaoundґe).
Nature of the aqueous nanostructures:
Treatments by RNAseA (Promega, 1 μg/ml, 37℃ 1 h), Dnase I (Invitrogen, 10 U/μg DNA, 37℃, 18 h), Lysozyme (Fisher, 1 mg/mL, 37℃ 10 min), Proteinase K (Promega, 0.12 mg/mL, in 1% sodium dodecyl sulphate, 56℃ 1 h) did not suppress the EMS producing activity of the “loud” dilutions nor did activate the “silent” dilutions. However, heating at 70℃ for 30 min suppressed irreversibly the activity, as well as did freezing for 1 hour at −20℃ or −60℃.
= замораживание равно нагреванию. Святая вода прямо
DNA extracted from the bacterial suspension by the classical phenol: chloroform technique was able upon filtration and appropriate dilutions in water to emit EMS similar to those produced by intact bacteria under the same conditions. DNAse treatment of the extracted DNA solution abolishes its capacity to emit signals, at the condition that the nanostructures previously induced by the DNA are destroyed.
in Tris 10-2 M, pH 7,6 and an aliquot was diluted 1/100 in water. The dilution (10−2) was filtered first through a 450 nM filter and the resulting filtrate was then filtered again on a 100 nM filter. The filtrate was further diluted in serial decimal dilutions in water as previously described. As for the intact microorganisms, the filtration step was found to be essential for detection of the EMS in the DNA dilutions. In its absence, no signals could be detected at any dilutions. In contrast to the microorganism suspension, where the filtration was supposed to retain the intact cells, the filtration at 100 nM did not retain the DNA, which was still present in the filtrate, as measured by optical density. However, filtration with a 20 nM Whatman filter retained the nanostructures emitting the EMS, suggesting that they have the same range of sizes than those originating from intact bacteria.
Treatment of the DNA solution by a restriction enzyme acting at many sites of E. Coli DNA (EcoRV) did not suppress the production of EMS, suggesting that this emission is linked to rather short sequences or is associated with rare sequences.
By contrast, probiotic “good” bacteria as Lactobacillus and their DNA are negative for EMS emission. In the case of E. Coli, we found that some strains used to carry plasmids for gene cloning were also negative
= это кирдык эффекту в принципе … -
we focussed our analysis again to M. pirum DNA, where a single gene (adhesin: 126-kDa protein) is responsible for the adhesion of the mycoplasma to human cells. our laboratory (Tham et al., 1994).
The cloned DNA existed as two fragments in two plasmids, corresponding respectively to the N terminal (1.5 Kbp) and the C terminal (5 Kbp) of the protein.
= опять путаница… - только 5 Kbp уже кодирует гораздо больший полипептид, чем 126-kD (\3435 bp 1145aa 126 kD\)
см их же ссылку - (www.ncbi.nlm.nih.gov/pmc/articles/PMC205115/) – там размер HindIII-XbaI фрагмента не 5kb – a прибл 2.5…
The two plasmids (pBluescript SK, Stratagene) containing the DNA fragments were amplified in a E. Coli strain, XL1blue. The DNA of the E. Coli strain (with or without the plasmid) alone did not yield EMS at any dilutions. By contrast when the strain was transformed with either plasmids carrying an adhesin gene fragment, EMS were produced
=опять мистика…
И особенно радует, то, что авторы запатентовали все это для практического применения в медицине.
= такое впечатление, что статью написал «студент»..
