Может быть взорвать в этой теме водородную бонбу ?

ты потерпи просто - она сама взорвется

Об этом нужно думать, но первично - это статистика (и при том не виртуальная). Именно это слабое место.

Админам предложение - повесить чукче за ЦУ медаль на... ну, сами найдете, куда.

Да не за что ) - только не пишите больше, что остановили работу из-за отстутствия денег на ПЦР...

Обратитесь в любую ПЦР-диагноститескую фирму (лучше сразу в несколько) и попрсите определить интересующую Вас последовательность в данном образце, причем именно в темную. Получите ответ с гарантией и без всяких соавторов - обычная платная услуга Примерно 100р за анализ.

А вот что имел ввиду чукча (Фантомы инвесторов маячат у порога и все суют суют суют...) - только ему и понятно...

Но для начала надо разобраться, какие факторы влияют на сам факт синтеза ДНК на водной матрице.

Я уж и забыл, что это за водная матрица?

...Ну, вот сахар и дрожжи у нас уже имеются, теперь нужна вода. Воду можно использовать водопроводную...

Но для начала надо разобраться, какие факторы влияют на сам факт синтеза ДНК на водной матрице. Температура? Расстояние до лазера? Время экспозиции? Время года? Концентрация ДНК-донора? Внешнее ЭМ окружение? И т.д.

В британских школах могут снова ввести физические наказания

Тем, кто в танке, разъясняю - последовательности нуклеотидов нас интересуют, конечно.

ППГ написал(а):
Тем, кто в танке, разъясняю - последовательности нуклеотидов нас интересуют, конечно.

Наша компания первая в России запускает секвенатор нового поколения Ion Torrent от life technologies.
С помощью него возможно осуществлять ресеквенирование геномов (поиск SNP, структурных перестроек и вариаций, полногеномное генотипирование). Направленное ресеквенирование отдельных крупных фрагментов геномов.

В несколько ведущих лабораториях мира идет проверка наших экспериментов по телепортации (вибропортации - добавлено мною). К концу года ожидается подтверждение наших результатов.

Из танка получен ответ ... для тех кто хочет ....

оже пояню, что просто ПЦРом определяют наличие данной в образце и ее количество, а не ее сиквенс.

К концу 2012 года?

И из другого танка -

Из танка получен ответ

Народ потихоньку перебирается в танки...

Также напомню, как прокололся Jacques Benveniste: ПЦР работал

Я уж и забыл, что это за водная матрица?

давлении в 40000 атмосфер

Quantrinas написал...
и какое отношение это имеет к ДНК?

и какое отношение это имеет к ДНК?

и какое отношение это имеет к ДНК?

'...We initially observed (Montagnier and Lavallee, personal communication) that some filtration procedures aimed at sterilizing biological fluids can yield under some defined conditions the infectious microorganism which was present before the filtration step. Thus, filtration of a culture supernatant of human lymphocytes infected with Mycoplasma pirum, a microorganism of about 300 nM in size, through filters of 100 nM or 20 nM porosities, yielded apparently sterile fluid. The latter however was able to regenerate the original mycoplasma when incubated with a mycoplasma negative culture of human lymphocytes within 2 to 3 weeks....'

‘…In addition to M. pirum, a more classical bacterium, E. Coli, was utilized for the purpose of the analysis….M. pirum is a peer-shaped small bacterial cell, ressembling M. pneumoniae, which can be grown in synthetic enriched medium (SP4) (Tully et al., 1977) but also mutiplies at the surface of human T lymphocytes. The strain (Ber) used in our experiments was isolated from a T lymphocyte culture derived from the blood of an apparently healthy subject (Grau et al., 1993). The strong mycoplasma adherence to lymphocytes is mediated by a specific adhesin, whose gene had been previously cloned and sequenced by the authors (Tham et al., 1994). We used as primary source of the mycoplasma, supernatants of infected human T lymphocyte cultures or of cultures of the CEM tumor T cell line. All cell cultures were first tested for the lack of M. pirum contamination by polymerase chain reaction (PCR) and nested PCR, before starting the experiments. Titers of 106-107 infectious Units/ml of M. pirum were readily achieved after 5-6 days of incubation following deliberate infection of both types of cultures….’

’…Filtration of the clarified supernatant was first performed on 0.45 M (450 nM) Millipore filters to remove debris, and subsequently on 0.1 M (100 nM) Millipore filters or on 0.02 M (20 nM) Whatman filters, to remove mycoplasma cells. Indeed, the two 100 nM and 20 nM filtrates were confirmed sterile when aliquots were incubated for several weeks in SP4 medium. Repeated search for traces of mycoplasma DNA by PCR and nested PCR using specific primers for the adhesion gene or for the 16S ribosomal gene was consistently negative. However when the filtrates were incubated for two weeks (100 nM filtrate) or three weeks (20 nM filtrate) with a culture of human activated T lymphocytes, the mycoplasma was recovered in the medium with all its original characteristics as previously observed…’

’…The story started ten years ago when one of us (L.M.) studied the strange behaviour of a small bacterium, a frequent companion of HIV, Mycoplasma pirum, and like HIV a lover of human lymphocytes. L.M. was trying to separate the bacterium, which is about 300 nm in size, from viral particles whose size is about 120 nm by filtration using filters of 100 nm and 20 nm. Starting with pure cultures of the bacterium on lymphocytes, the filtrates were indeed sterile for the bacterium when cultured on a rich cellular medium, SP4. Polymerase chain reaction (PCR) and nested PCR, based on primers derived from a gene of M. pirum which had been previously cloned and sequenced, adhesin, were negative in the filtrate.
However, when the filtrate was incubated with human lymphocytes, (previously controlled for not being infected with the mycoplasma) the mycoplasma with all its characteristics was regularly recovered!…’

Насколько я могу судить из прочитанного мною, вода, в которой ранее находились микроорганизмы была отфильтрована таким образом, что являлась стерильной. Однако после того когда с данной водой в течении определенного времени контактировали лимфоциты человека в этой воде появились находившиеся до процесса фильтрования микроорганизмы.

Мне кажется, это ещё Пастер опроверг

он не работал с ПЦРом...

Research team
The research team who conducted the research published by Nature was led by Professor Jacques Benveniste of the highly regarded French National Institute for Scientific Research in Medicine (INSERM--the equivalent to America's National Institutes of Health). Dr. Benveniste is a leading allergist and immunologist who declined President Mitterand's appointment to head this French research institute. This research was replicated by scientists at the Hebrew University, University of Toronto, and University of Milano.

Research overview
The researchers utilized the Human Basophil Degranulation Test, which is commonly performed in Europe but is virtually unknown in the U.S. Basophils are a kind of white blood cell which play an important role in the allergic response, and this test helps to determine to which substances a person is allergic.
Basophils contain granules which are made of histamine and other chemicals which can elicit symptoms of allergy when they are released or degranulated. A substance to which a person is allergic causes degranulation of the basophils.
There are certain substances which will cause anybody's basophils to degranulate. IgE is a special type of antibody which causes the release/degranulation of basophils, and anti- IgE is an artificial (man-made) antibody can have a similar effect as IgE.
What was revolutionary about the research of Benveniste and his colleagues is that they diluted anti-IgE 1:10, then shook it vigorously, diluted it again 1:10, then shook it again vigorously. They repeated this process of serial dilution with shaking as many as 120 times. It was calculated that it was unlikely that there was even a single molecule of the original anti-IgE after diluting this solution just 14 times.
Homeopaths call the pharmaceutical procedure of dilution and succussion (vigorous shaking) to be potentization. For the past 200 years they have asserted that the microdoses maintain biological activity and clinical efficacy when they are potentized and if they are correctly prescribed.

Debunking the research
Nature published this research with the stipulation that they could send a team to observe a replication of the experiment. The team included Nature's editor John Maddox, magician James Randi, and anti-fraud investigator Walter Stewart.
Under their supervision, the experiment was first replicated three times without any blinding of the experimentors (an procedure that the original experimentors never utilized). All three experiments showed positive results. The fourth experiment blinded the person doing the counting of the basophils, and the results of this experiment were again very successful. The Nature team deemed this experiment invalid, though this criticism presumed that the blinded experimentor knew in advance which test group she was counting (an implausible critique).
The next three experiments blinded the person doing the counting and the person doing the pipetting. All three of these experiments did not show any difference between the microdoses and the control group. The Nature team immediately deemed that there is no evidence that the microdoses have biological action.

Problems with the debunking
1) The Nature team ignored one blinded experiment which showed the biological action of the microdoses.
2) The Nature team presumed to have disproved Benveniste's five year's worth work in two days of experiments.
3) The Nature team did not include an immunologist, and thus they did not know that white blood cells are not always sensitive to large doses of antibiotics, let alone microdoses of them.
4) The Nature team created a disruptive environment in the laboratory which was not conducive to scientific investigation. James Randi performed magic tricks during a crucial part of the experiment, making it difficult for the experimenters to perform their work, while Walter Stewart was acting so hysterically that he had to be asked several times to stop shouting by John Maddox and by Jacque Benveniste.
5) The original work showed that heating, freeze-thawing, or ultrasonation suppressed the activity of the highly diluted solutions. This fact suggest that the microdoses are sensitive to various external stresses and that a couple of experiments that do not prove its action may have some unknown factor inhibiting its action.

Future research
Dr. Benveniste is confident in this research, and he has predicted that it will be replicated yet again by other researchers. The French homeopathic pharmacy which funded Dr. Benveniste's work has let it be known that they are willing to fund replication of this study by independent investigators.
The serious threat that the microdose phenomena and homeopathy poses to science and medicine is clear and evident by the strong antagonism that the initial research created. The fact that the critics ignored and continue to ignore the numerous other studies which support the action of the microdoses is further evidence of this denial and ultimately of an unscientific attitude.

Это к вопросу о множественности Эвереттовских вселенных

Ну наследование по Ламарку (когда среда влияет на генотип) тоже опровергли а 15 лет назад появилась эпигенетика с ее эпигенетичеческим наследованием и все вернулось.